Section: New Results
Cell-to-cell ascidian embryo registration
Participants : Gaël Michelin, Grégoire Malandain.
This work is made in collaboration with Léo Guignard and Christophe Godin (Virtual Plants) and Patrick Lemaire (CRBM), within the Morphogenetics Inria Project Lab.
Recent microscopy techniques allow imaging temporal 3D stacks of developing organs or embryos with a cellular level of resolution and with a sufficient acquisition frequency to accurately track cell lineages. Imaging multiple organs or embryos in different experimental conditions may help to decipher the impact of genetic backgrounds and environmental inputs on the developmental program. For this, we need to precisely compare distinct individuals and to compute population statistics. The first step of this procedure is to develop methods to register individuals.
From a previous work of cell segmentation from microscopy images [6] , we propose an approach to extract the Left-Right symmetry plane of embryos at early stages (Figure 7 ). Then we use the symmetry information to both register these embryos at a similar developmental stage and obtain a cell-to-cell mapping. We assessed the symmetry plane extraction on more than 100 images from 10 individuals between 32-cells and late-neurula development stage. The cell-to-cell registration was performed on 5 distinct individuals at 64-cells and 112-cells stage (Figure 8 ).
|
|