Section: New Results

Comparison of normalisation procedures in RNA-sequencing before differential analysis

Participant : Guillemette Marot.

The continuing technical improvements and decreasing cost of next-generation sequencing technologies have made RNA sequencing (RNA-seq) a popular choice for gene expression studies in recent years. Because the data collected from such studies differ considerably from those measured using microarray technology, the statistical tools used for analysis must be adapted accordingly. In particular, several methods for the normalization of RNAseq data (removal of errors due to the small number of samples, corrections for sequence composition) have been proposed in recent years. With the Statomique Consortium, we have compared seven normalisation methods. First results are given in [28] .