Section: New Results

Comparison of normalisation procedures in RNA-sequencing before differential analysis

Participant : Guillemette Marot.

The continuing technical improvements and decreasing cost of next-generation sequencing technologies have made RNA sequencing (RNA-seq) a popular choice for gene expression studies. Several methods for the normalization of RNAseq data (removal of errors due to the small number of samples, corrections for sequence composition) have been proposed in recent years. With the Statomique Consortium, we have compared seven normalisation methods, discarded two out of them (although still widely used). We give practical recommendations on the appropriate normalization method to be used and its impact on the differential analysis of RNA-seq data in the paper ([14] ).