Section: New Results
Exact continuous penalties for - minimization: Application to Photo Activated Localization Microscopy (PALM)
Participants : Simon Gazagnes, Emmanuel Soubies, Laure Blanc-Féraud.
In conventional microscopy techniques, the spatial resolution of an image is limited by the diffraction phenomena. Recent methods like photo-activated localization microscopy (PALM) allow high-precision molecule localization by sequentially activating and imaging a small random set of fluorescent molecules in the sample. However, the quality of this super-resolved image is related to the density of emitters activated at each acquisition and the numerical method used to locate molecules.
Applications for these microscopy techniques are then mainly
restricted by the number of acquisitions required to obtain the
superresolved image. One way to overcome
this limitation is to increase the density of emitters activated at
each acquisition. Nevertheless, it will cause overlapping for a
certain number of spots on the acquired image which makes the
localization of the underlying molecules a harder task. Considering
such a high density setting, we have proposed to perform the molecules
localization by solving a