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Section: New Results

Reconstruction of mosaic of microscopic images

Participants : Kévin Giulietti, Eric Debreuve, Grégoire Malandain.

This work takes place within the ANR PhaseQuant.

In microscopy imaging, a trade-off has to be made between a high resolution, that enables to see details, and the width of the field of view, that enables to see many objects. Such a trade-off is avoided by mosaicing, which consists in the acquisition of several images, say $N\times N$, with a small overlap between images. This way, an image with a $N$ larger field of view can be reconstructed with the same resolution than a single microscopic image.

Such an imaging protocol is available on many microscopy software. Basically, displacements of the table on which lies the material to be imaged are programmed, and used to reconstruct the mosaic. However, it appears (at the overlapping areas), that a residual offset is still present. The cause of this has not be identified so far: this may be due to small geometric mis-alignement in the imaging device, or to the command of the micrometer table.

We thus investigate the stability of this residual offset with respect to time and to the image position within the mosaic.