Section: Research Program

From models to image data: simulation and modelling of membrane transport

Mathematical biology is a field in expansion, which has evolved into various branches and paradigms to address problems at various scales ranging from ecology to molecular structures. Nowadays, system biology [44] , [59] aims at modelling systems as a whole in an integrative perspective instead of focusing on independent biophysical processes. One of the goals of these approaches is the cell in silico as investigated at Harvard Medical School (http://vcp.med.harvard.edu/ ) or the VCell of the University of Connecticut Health Center (http://www.nrcam.uchc.edu/ ). Previous simulation-based methods have been investigated to explain the spatial organization of microtubules [47] but the method is not integrative and a single scale is used to describe the visual patterns. In this line of work, we propose several contributions to combine imaging, traffic and membrane transport modelling in cell biology.

In this area, we focus on the analysis of transport intermediates (vesicles) that deliver cellular components to appropriate places within cells. We have already investigated the concept of Network Tomography (NT) [58] mainly developed for internet traffic estimation. The idea is to determine mean traffic intensities based on statistics accumulated over a period of time. The measurements are usually the number of vesicles detected at each destination region receiver. The NT concept has been investigated also for simulation [3] since it can be used to statistically mimic the contents of real traffic image sequences. In the future, we plan to incorporate more prior knowledge on dynamics to improve representation. An important challenge is to correlate stochastic, dynamical, one-dimensional in silico models studied at the nano-scale in biophysics, to 3D images acquired in vivo at the scale of few hundred nanometers. A difficulty is related to the scale change and statistical aggregation problems (in time and space) have to be handled.