Section: New Software and Platforms

THOTH

Testing HypOtheses for diffusion TricHotomy

Keywords: Photonic imaging - Fluorescence microscopy - Biomedical imaging - Classification - Statistical categorisation techniques - Statistics - Image sequence - Visual tracking

Functional Description: The THOTH software classifies biomolecule trajectories of biomolecules (computed with tracking algorithms) into three groups of diffusion: (i) free diffusion, (ii) subdiffusion or (iii) superdiffusion. Brownian motion corresponds to the NULL hypothesis. THOTH is a nonparametric three-decision test whose alternatives are subdiffusion and superdiffusion. Single and multiple testing procedures control respectively the type I error and the false discovery rate. THOTH can be considered as an alternative to the Mean Square Displacement (MSD) method commonly used to address this issue. It gives more reliable results as confirmed by our Monte Carlo simulations and evaluations on real sequences of images depicting protein dynamics acquired with TIRF or SPT-PALM microscopy.

• Participants: Vincent Briane, Charles Kervrann and Myriam Vimond

• Partner: ENSAI

• Contact: Charles Kervrann

• Publication: Statistical analysis of particle trajectories in living cells

• URL: https://team.inria.fr/serpico/software/thot/