Section: New Results

Repetitive and transient event detection in fluorescence video-microscopy

Participants : Charles Kervrann, Pierre Hellier.

Progresses in imaging dynamic behaviours of molecules including fast video microscopy and the application of evanescent wave microscopy have allowed to image intracellular vesicular movements, exocytosis and endocytosis of fluorescent-tagged proteins. For an unbiased quantification of repetitive and transient events, we have proposed an approach which is versatile enough, to be applicable to diverse although complementary modes of microscopy. The proposed detection method described in [21] , [11] , [16] can be decomposed into three main steps: i) a first pre-processing step is dedicated to the normalization of the image sequence; ii) the second step is the patch-based detection procedure to detect unusual patterns; iii) a third post-processing step allows us to cluster and count detected events in space and time. In a more recent case study, we have used this approach to analyse image sequences depicting M10 cells stably expressing Langerin-YFP and to get deeper insights in the recycling pathway and dynamics of this molecule.

Partners:: A. Gidon, B. Cinquin, A. Chessel, J. Boulanger and J. Salamero (UMR 144 CNRS Institut Curie)