Section: New Results

Atlas creation of fluorescence microscopy images

Participant : Pierre Hellier.

In this work, we consider the analysis of fluorescence images over time to account for two artifacts: fluorescence decreasing over time and geometric misalignment. A single exponential function is most commonly used to represent measured fluorescence decay profiles due to photobleaching. Accordingly, homologous points need to be geometrically aligned over time. Unfortunately the living cell exhibits slow motion over time. We have considered the iterative estimation of both geometrical alignment and intensity correction by the creation of a 3D atlas.