Section: Scientific Foundations
Glossary
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Glossary
- WF
Optical Wide-Field microscopy.
- SDC
(Spinning-Disk Confocal microscopy): illumination of the sample with a rotating pattern of several hundred of pinholes for complete simultaneous confocal illumination.
- FLIM
(Fluorescence Lifetime Microscopy Imaging): imaging of fluorescent molecule lifetimes.
- PALM
(Photo-Activated Localization Microscopy): high-resolution microscopy using stochastic photo-activation of fluorophores and adjustment of point spread functions [20] .
- SIM
(Structured Illumination Microscopy): high-resolution light microscopy using structured patterns and interference analysis [30] .
- TIRF
(Total Internal Reflectance): 2D optical microscopy using evanescent waves and total reflectance [19] .
- Cryo-EM
(Cryo-Electron Tomography): 3D representation of sub-cellular and molecular objects of 5-20 nanometres, frozen at very low temperatures, from 2D projections using a transmission electron microscope.