Section: New Software and Platforms

TOTH

Keywords: Photonic imaging - Fluorescence microscopy - Biomedical imaging - Classification - Statistical categorisation techniques - Statistics - Image sequence - Visual tracking

Functional Description: The TOTH software classifies trajectories of biomolecules computed with tracking algorithms. Trajectories in living cells are generally modelled with three types of diffusion processes: (i) free diffusion, (ii) subdiffusion or (iii) superdiffusion. We used a test approach with the Brownian motion as the null hypothesis, and developed a non-parametric three-decision test whose alternatives are subdiffusion and superdiffusion. First, we built a single test procedure for testing a single trajectory. Second, we proposed a multiple test procedure for testing a collection of trajectories. These procedures control respectively the type I error and the false discovery rate. Our approach can be considered as an alternative to the Mean Square Displacement (MSD) method commonly used to address this issue. It gives more reliable results as confirmed by our Monte Carlo simulations and evaluations on real sequences of images depicting protein dynamics acquired with TIRF or SPT-PALM microscopy.

• Participants: Vincent Briane and Myriam Vimond

• Partner: ENSAI

• Contact: Charles Kervrann

• Publication: A Statistical Analysis of Particle Trajectories in Living Cells

• URL: http://serpico.rennes.inria.fr/doku.php?id=software:thot:index