Section: New Results

Empirical SURE-guided microscopy super-resolution image reconstruction from confocal multi-array detectors

Participants : Sylvain Prigent, Charles Kervrann.

Recent confocal microscopes use an array detector instead of single point detector to take multiple views of the same sample. The microscope output is then an array of images, one image per detector. The array of images is then processed to build a single image with higher signal-to-noise ratio and higher resolution than a classical confocal microscope image. In the literature, several methods have been recently proposed to reconstruct the single high resolution image: i/ the ISM method combines array registration and Wiener deconvolution; ii/ the IFED method estimates a high resolution image by subtracting the outer detectors of the array to the inner detectors; iii/ the ISFED consists in subtracting the outer registered detectors and the outer raw images. In that context, we proposed a SURE-guided (Stein’s unbiased risk estimation) estimation method to automatically select the parameter $ϵ$ controlling the IFED and ISFED reconstruction algorithms (see Figure 4). We showed on real data that the proposed method is capable to achieve a resolution close to 100 nm without any deconvolution method.

Software: AiryscanJ (see Section 6.4).

Collaborator: S. Dutertre (IGDR – Institute Genetics & Development of Rennes).

Figure 4. Results obtained on a c.elegans sample and intensity profiles for all the tested methods. The profile line is shown with a red line on the ISM-DS image (top right).